Fig. 6. LPIN1 is a critical target during miR-451a-induced SMMC-7721 cell apoptosis. A, SMMC-7721 cells were transfected with an LPIN1 expression plasmid in the presence or absence of miR-451a mimics. Apoptosis was determined by Annexin V assays. B, SMMC-7721 cells were transfected with specific LPIN1-targeting shRNAs in the presence or absence of miR-451a mimics. Apoptosis was determined by Annexin V assays. ns, not significant; NC, negative control; SC, shRNA control; *P<0.05, **P<0.01.